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  • Küçük Resim Yok
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    Collagen-carrageenan-chitosan sponge reinforced with 3D-printed polycaprolactone mesh for skin tissue engineering
    (Wiley, 2026) Yesiloglu, Buket; Barer, Neslihan; Baran, Eray A.; Serin, Erdal; Dalgic, Ali Deniz; Erdemli, Bengi Yilmaz; Tahmasebifar, Aydin
    Biopolymers have gained prominence due to their potential in tissue engineering, which includes hydrophilicity, lower toxicity, reduced immune rejection, biocompatibility and biodegradability. However, biopolymers typically exhibit low mechanical strength, which decreases their application potential in tissue engineering. Composites of natural and synthetic polymers offer a robust solution to overcome this challenge, as the stiffness and durability of composites are enhanced by the presence of synthetic polymers. This study investigates two composite sandwich model scaffolds for skin tissue engineering, focusing on their structural and regenerative properties. The composite scaffolds were fabricated by combining freeze-drying and 3D-printing techniques. The outer layers of the scaffolds were fabricated using collagen-carrageenan (CO/CA-PCL) or collagen-carrageenan-chitosan (CO/CA/CH-PCL) through freeze drying, whereas the core layer was formed by 3D-printed polycaprolactone (PCL) mesh. Crosslinking was achieved in the vapor phase of glutaraldehyde and scaffold groups preserved their structure through 28 days after an initial weight loss on day 1. The CO/CA/CH-PCL scaffold showed a lower degradation rate with a cumulative weight loss of 28.6 +/- 5.5% compared to the CO/CA-PCL scaffold which indicates improved stability of the three-polymer sponge. Both scaffolds achieved water retention above 800% after 14 days of incubation which is critical for wound healing. Tensile strength of both scaffolds was successfully supported by 3D-printed PCL mesh. In vitro study has shown that the chitosan-bearing CO/CA/CH-PCL scaffold is promising for use in skin tissue engineering by supporting L929 attachment and high L929 cell viability. (c) 2025 Society of Chemical Industry.
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    Development of a hydrogel system to sustain local microsphere release for post-operative treatment of breast cancer
    (İstanbul Bilgi Üniversitesi, 2025) Barer, Neslihan; Dalgıç, Ali Deniz
    Meme kanseri hastalarında cerrahi müdahale sonrası tedavi yoluyla erken müdahale, lokal bölgesel tekrarı veya kalan mikrotümör hücrelerinin metastazını önlemek için önemlidir. Geleneksel ilaç verme sistemleri genellikle bu gereklilikleri karşılamada yetersiz kaldığından, bölgeye özgü tedavilere yönelik klinik talep oldukça önem kazanmıştır.Bu doğrultuda, bu çalışma, cerrahi müdahale sonrası meme kanseri tedavisi için tasarlanmış, doksorubisinin (DOX) lokalize iletimini kolaylaştıran yeni bir poli (vinil alkol)/pullulan (PVA/PUL) hidrojel platformu sunmaktadır. Birincil taşıyıcı platform olan hidrojel, DOX kapsüllenmiş polikaprolakton mikroküreleri (DOX- PCL- MS'ler) için rezervuar görevi gören birbirine bağlı mikro ve makro gözenekler oluşturan liyofilizasyon ve buz kabartma teknikleri olmak üzere iki birleşik yöntem kullanılarak üretilmiştir ve kontrollü ve uzun süreli ilaç salımını mümkün kılmıştır. Glutaraldehit (GA) çapraz bağlı, stabilize edilmiş PVA/PUL hibrit hidrojel sistemi, su tutma kapasitesi ve yavaş bir degradasyon profili göstermiştir. Hidrojelden doğrudan salım ve DOX-PCL-MS'leri içeren hidrojel olarak farklı platformlardan DOX salımı incelendiğinde, DOX-PCL-MS'leri içeren hidrojelin gecikmeli bir salım sergilediği, ani bir salım olmadan %56.6 ± 4.5'e ulaştığı, buna karşın hidrojelden doğrudan DOX salımının %76.79 ± 19.72 ile sonuçlandığı gözlemlenmiştir. Ek olarak, hem tek katmanlı hem de sferoid formlarda insan meme kanseri hücre hattı MCF-7 kullanılarak in vitro çalışmalar yürütülmüş ve sonuçlar her iki hücre morfolojisinin de 12,5 ?g/mL kadar düşük DOX-PCL-MS konsantrasyonlarında dahi bozulduğunu göstermiştir. Yedi günlük inkübasyon sürecinden sonra ise MCF-7 hücre canlılığı önemli ölçüde azalmıştır, serbest DOX yüklü hidrojelin ise 4. günde sitotoksik etkinliğinde azalma gözlemlenmiştir.Hibrit hidrojel sistemlerinin sito-uyumluluğu L929 fibroblast hücreleriyle de araştırılmıştır; hidrojelin hücrelerin yapışmasını desteklediği gözlelenmiştir. DOX-PCL-MS'leri içeren PVA/PUL hidrojeline ilişkin bu bulgular, sistemin meme kanserinin cerrahi müdahale sonrası tedavisi için hedefe yönelik ve kontrollü salım sağlayan ilaç taşıma sistemi olarak potansiyelini vurgulamıştır.
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    Using embossing ice particulate method to prepare polyvinyl alcohol/ pullulan hydrogels with surface open pores loaded with microspheres for breast cancer treatment
    (Elsevier, 2024) Barer, Neslihan; Tunc, Bugse; Yilmaz, Bengi; Ng, Yuk Yin; Dalgic, Ali Deniz
    In the post -operative treatment of breast cancer, early prevention of locoregional recurrence is crucial to avoid metastasis of cells from leftover microtumor tissues. The limitations in conventional drug delivery systems show a growing clinical demand for disease -specific drug -releasing systems. This study explores a novel poly(vinyl alcohol)/pullulan (PVA/PUL) hydrogel system for local drug delivery in post -operative breast cancer treatment. Hydrogel was produced by combination of lyophilization and embossing ice particulate techniques to create microspheres loaded open pores on the hydrogel surface for localized release of doxorubicin-loaded polycaprolactone microspheres (DOX-PCL-MSs). PVA/PUL hydrogel was successfully crosslinked with glutaraldehyde and stabilized hydrogel structure has possessed slow degradation rate and increasing water retention through 12 days. Release of DOX after 7 and 16 days from DOX-PCL-MSs loaded hydrogels were slower with a 6.2 +/- 8.9 % and 56.6 +/- 4.5 % release compared to 60.9 +/- 14.6 % and 76.8 +/- 19.7 % release from free DOX loaded hydrogel since DOX release was controlled by PCL microspheres. When interacted with human breast cancer cell line (MCF-7), DOX-PCL-MSs were able to disrupt cell and spheroid morphology after 7 days at concentrations as low as 12 mu g/mL loaded DOX. In vitro cytotoxicity study has showed that, DOX-PCL-MSs loaded hydrogel was able to decrease MCF-7 viability after 7 days of incubation with controlled release of DOX. While free DOX releasing hydrogel has lost cytotoxic activity even after 4 days of incubation. Furthermore, ability of PVA/PUL hydrogel to support L929 cell attachment was shown in the study, suggesting hydrogels potential for promoting tissue regeneration after anti -cancer treatment. The study reveals that PVA/PUL hybrid hydrogels loaded with DOXPCL-MSs has impactful potential in post -surgical treatment of breast cancer.