The influence of selenium on expression levels of the rbcL gene in Chlorella vulgaris

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dc.WoS.categoriesBiotechnology & Applied Microbiologyen_US
dc.authorid0000-0003-1001-939Xen_US
dc.contributor.authorÖzakman, Gülru
dc.contributor.authorYayman, Sinem Gamze
dc.contributor.authorZhmurov, Çiğdem Sezer
dc.contributor.authorKaşıkçı, Emel Serdaroğlu
dc.contributor.authorÇatal, Tunç
dc.date.accessioned2020-11-12T08:51:50Z
dc.date.available2020-11-12T08:51:50Z
dc.date.issued2018-03-20
dc.description.abstractIn this study, the effects of selenium on the microalgae Chlorella vulgaris were examined. Four groups of C. vulgaris were cultivated using Bristol medium: group I (control), no sodium selenite (Se); group II, 1 mu M Se; group III, 10 mu M Se; and group IV, 100 mu M Se. Algal biomass samples were collected for biochemical evaluation and gene expression studies on the 21st day of cultivation. The following parameters were investigated: chlorophyll a (Cl-a), chlorophyll b (Cl-b) and total carotene content, total protein, and total glutathione (GSH) and malondialdehyde (MDA) levels. Gene expression levels of large subunits of Rubisco (rbcL) were analyzed using real-time quantitative polymerase chain reaction. Total Cl-a and total carotene in C. vulgaris decreased in high concentrations of Se (100 mu M) (around 23 and 42%, respectively) when compared to controls while, Cl-b content increased by about 10%. 10 mu M of Se led to increased GSH levels (3.04 +/- 0.02 mu g GSH/mg protein) and decreased MDA levels (2.02 +/- 0.1 mu mol MDA/mg protein) when compared to control groups (1.18 +/- 0.04 mu g GSH/mg protein and 0.94 +/- 0.23 mu mol MDA/mg protein), while a significant decrease in GSH and an increase in MDA levels in the presence of 100 mu M Se showed the opposite effect. rbcL gene expression increased 1.76 +/- 1.37-fold and 0.86 +/- 1.33-fold in 10 and 100 mu M selenium experiments when compared to control groups. Our results suggest both pro-oxidant and antioxidant activities of Se on C. vulgaris and upregulation of the rbcL gene for the first time. Treatment with low concentrations of Se improves the antioxidant features of the microalgae, C. vulgaris.en_US
dc.fullTextLevelFull Texten_US
dc.identifier.doi10.1007/s13205-018-1212-4en_US
dc.identifier.issn2190-5738
dc.identifier.issn2190-572X
dc.identifier.pmid29564200en_US
dc.identifier.scopus2-s2.0-85044311502en_US
dc.identifier.urihttps://hdl.handle.net/11411/2610
dc.identifier.urihttps://doi.org/10.1007/s13205-018-1212-4
dc.identifier.wosWOS:000428183500001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.issue4en_US
dc.language.isoenen_US
dc.nationalInternationalen_US
dc.numberofauthors5en_US
dc.publisherSPRINGER HEIDELBERGen_US
dc.relation.ispartof3 BIOTECHen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBiotechnologyen_US
dc.subjectChlorella vulgarisen_US
dc.subjectMalondialdehydeen_US
dc.subjectrbcL gene expressionen_US
dc.subjectSeleniumen_US
dc.titleThe influence of selenium on expression levels of the rbcL gene in Chlorella vulgarisen_US
dc.typeArticleen_US
dc.volume8en_US

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