A disposable MEMS biosensor for aflatoxin M1 molecule detection

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dc.authoridAkcan, Omer Gokalp/0000-0002-4374-1656|Erdil, Kuter/0000-0003-4988-1913
dc.contributor.authorErdil, Kuter
dc.contributor.authorAkcan, O. Gokalp
dc.contributor.authorGul, Ozguer
dc.contributor.authorGokdel, Y. Daghan
dc.date.accessioned2024-07-18T20:56:05Z
dc.date.available2024-07-18T20:56:05Z
dc.date.issued2022
dc.departmentİstanbul Bilgi Üniversitesien_US
dc.description.abstractIn this work, a paper-based perforated disposable biosensing device is proposed as an alternative method for aflatoxin M1 molecule detection. The demonstrated system is designed to achieve a quick and novel biosensing operation with low-cost materials using competitive assay method. For that purpose, the main fabrication material has opted as a 190 izm thick filter paper. 50 izm thick piezoresistive graphite paste is coated onto both sides of the paper-based cantilever beam with the aim of acquiring more sensitive magnetic nanoparticle weight sensing capability. Additionally, the structure has arrays of closely spaced perforations to augmented effective Young's modulus of the cantilever beam and further increase the system's sensitivity. An electrocoil positioned 1 mm below the sensor tip to apply an H(ext )and magnetically increase weight of the aflatoxin M1 with bovine serum albumin compound. An electronic read-out circuitry is implemented and integrated into the system. Average values of sensitivity and limit of detection (LoD) for each detection approach were calculated without blank subtraction and are shown with the standard error of the mean (SEM). LoD is calculated as 4.63 izg AFM1 which corresponds to 0.20127 V/V after subtracting standard deviation from the average value. It is experimentally demonstrated that the proposed system can detect a minimum of 14 izg of AFM1 molecules (0.14155 V/V). We magnetically amplified this tiny fragment of targeted molecules approximately 2731 times to 38.237 mg and made it detectable even with a disposable system. The sensitivity of the proposed system is 45.953 izV/mg. Finally, the maximum detectable AFM1 weight is reported as 71 izg.en_US
dc.description.sponsorshipTUBITAK EEEAG [117E236]; TUBITAKen_US
dc.description.sponsorshipThis work is funded by TUBITAK EEEAG with a project number of 117E236. The authors would like to sincerely thank to TUBITAK and research group members Oznur Mete, Tugce Ayrac, and Elif Teker.en_US
dc.identifier.doi10.1016/j.sna.2022.113438
dc.identifier.issn0924-4247
dc.identifier.issn1873-3069
dc.identifier.scopus2-s2.0-85125741149en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.sna.2022.113438
dc.identifier.urihttps://hdl.handle.net/11411/8861
dc.identifier.volume338en_US
dc.identifier.wosWOS:000797593000002en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Science Saen_US
dc.relation.ispartofSensors and Actuators A-Physicalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPiezoresistive Sensingen_US
dc.subjectCompetitive Assayen_US
dc.subjectBiomolecule Sensingen_US
dc.subjectMagnetic Amplificationen_US
dc.subjectAflatoxinen_US
dc.titleA disposable MEMS biosensor for aflatoxin M1 molecule detectionen_US
dc.typeArticleen_US

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