Decellularization and characterization of leek: a potential cellulose-based biomaterial

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dc.authoridrostami, sabra/0000-0002-2687-1192;
dc.authorwosidKesici, Müge/ABD-6710-2020
dc.authorwosidrostami, sabra/AAW-4855-2020
dc.authorwosidGaripcan, Bora/AAS-9920-2020
dc.authorwosidGul, Ozgur/A-7841-2011
dc.contributor.authorToker, Melis
dc.contributor.authorRostami, Sabra
dc.contributor.authorKesici, Muge
dc.contributor.authorGul, Ozgur
dc.contributor.authorKocaturk, Ozgur
dc.contributor.authorOdabas, Sedat
dc.contributor.authorGaripcan, Bora
dc.date.accessioned2024-07-18T20:40:39Z
dc.date.available2024-07-18T20:40:39Z
dc.date.issued2020
dc.departmentİstanbul Bilgi Üniversitesien_US
dc.description.abstractCellulose, which is easily attainable in nature, has been studied due to its biocompatibility, low cytotoxicity, adjustable biomechanical properties and cost effectiveness as a biomaterial. Recently, plant-derived cellulose-based biomaterials were given attention for numerous applications including bone, cartilage and cardiac tissue engineering. In this study, leek (Allium porrum) was chosen as a plant tissue model for the fabrication of a potential biomaterial due to its structural morphology (interconnected and elongated channel like structural morphology). Leek tissues were decellularized by a detergent solution. The degree of residual cell content was evaluated by DNA and protein quantification as well as immunostaining. Chemical and mechanical properties were tested for both native and decellularized leek samples in order to investigate the effect of decellularization on the structure. Swelling, degradation and protein adsorption behavior of decellularized leek samples were also studied. In order to enhance cell adhesion, decellularized leek samples were modified with 3-aminopropyltriethoxysilane, octadecyltrichlorosilane and coated with graphene oxide prior to cell seeding. SH-SY5Y human neuroblastoma cells were used for mammalian cell culture studies. MTT cell viability assay and SEM imaging were performed to observe the cell adhesion and morphology. Decellularized leek tissues are expected to be cellulose based biomaterial for candidate biomedical applications both in vitro and in vivo in future studies. Graphic abstracten_US
dc.description.sponsorshipBogazici University Research Fund [6701]en_US
dc.description.sponsorshipThe authors would like to thank Deniz Bayraktar for conducting mechanical tests. This work is partially supported by Bogazici University Research Fund Grant Number No: 6701.en_US
dc.identifier.doi10.1007/s10570-020-03278-4
dc.identifier.endpage7348en_US
dc.identifier.issn0969-0239
dc.identifier.issn1572-882X
dc.identifier.issue13en_US
dc.identifier.scopus2-s2.0-85086832866en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage7331en_US
dc.identifier.urihttps://doi.org/10.1007/s10570-020-03278-4
dc.identifier.urihttps://hdl.handle.net/11411/7162
dc.identifier.volume27en_US
dc.identifier.wosWOS:000543127800001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofCelluloseen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLeek (Allium Porrum)en_US
dc.subjectPlantsen_US
dc.subjectCelluloseen_US
dc.subjectDecellularizationen_US
dc.subjectSurface Modificationen_US
dc.subjectBiomaterialsen_US
dc.subjectGraphene Oxideen_US
dc.subjectSurface Modificationen_US
dc.subjectFibersen_US
dc.subjectDegradationen_US
dc.subjectProteinsen_US
dc.subjectAlbuminen_US
dc.subjectInsighten_US
dc.subjectWooden_US
dc.titleDecellularization and characterization of leek: a potential cellulose-based biomaterialen_US
dc.typeArticleen_US

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