Natural origin bilayer pullulan-PHBV scaffold for wound healing applications

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dc.authoridKOMAN, EZGI/0000-0001-5530-3314|Dalgıç, Ali Deniz/0000-0003-2904-1204|Yazgan-Karatas, Ayten/0000-0003-0160-3914|/0000-0001-7824-488X|Tezcaner, Aysen/0000-0003-4292-5856
dc.authorwosidKOMAN, EZGI/GWZ-1709-2022
dc.authorwosidDalgıç, Ali Deniz/AAZ-8985-2020
dc.authorwosidYazgan-Karatas, Ayten/D-2854-2014
dc.contributor.authorDalgic, Ali Deniz
dc.contributor.authorKoman, Ezgi
dc.contributor.authorKaratas, Ayten
dc.contributor.authorTezcaner, Aysen
dc.contributor.authorKeskin, Dilek
dc.date.accessioned2024-07-18T20:55:15Z
dc.date.available2024-07-18T20:55:15Z
dc.date.issued2022
dc.departmentİstanbul Bilgi Üniversitesien_US
dc.description.abstractSkin tissue loss that occurs by injury and diseases can turn into chronic wounds as a result of complications alongside infection. Chronic wounds fail to heal by themselves and need advanced treatments like engineered wound dressings and regenerative scaffolds. In this study, a novel, natural origin, bilayer electrospun scaffold was produced from pullulan (PUL) and poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) biopolymers. PHBV production by Cupriavidus necator bacterial strain was optimized and produced polymer was characterized. Characteristic peaks and bands of PHBV were observed by H-NMR and FTIR analyses. Valerate mol percent of produced PHBV copolymer was determined by H-NMR. Average molecular weight of the polymer was determined by SLS technique and crystallinity of PHBV was calculated from DSC curve. Bilayer scaffold was produced by electrospinning of hydrophilic PUL fibrous membrane onto wet-electrospun hydrophobic PHBV 3D fibrous mat. Bilayer scaffold was designed to involve regenerative and barrier fibrous layers. Nano fibrous PUL membrane with smaller pore size was efficient as a barrier against bacterial transmission while enabling optimum oxygen and water vapor transmission. Water retention and degradation properties were found to be optimum for a skin tissue scaffold. In vitro studies showed that PUL membrane sustained L929 cell proliferation while preventing cells from migrating inside the barrier phase while PHBV layer supported cell viability, proliferation, and migration, creating a regenerative 3D structure. Results showed that, novel natural origin PUL/PHBV bilayer scaffold is a promising candidate for wound healing applications.en_US
dc.description.sponsorshipTUBITAK [215M893]en_US
dc.description.sponsorshipAuthors want to acknowledge that production and characterization of PHBV was optimized in the TUBITAK project 215M893.en_US
dc.identifier.doi10.1016/j.msec.2021.112554
dc.identifier.issn2772-9508
dc.identifier.pmid35523643en_US
dc.identifier.scopus2-s2.0-85183076020en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttps://doi.org/10.1016/j.msec.2021.112554
dc.identifier.urihttps://hdl.handle.net/11411/8801
dc.identifier.volume134en_US
dc.identifier.wosWOS:000811742400002en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofBiomaterials Advancesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPhbven_US
dc.subjectPullulanen_US
dc.subjectWet-Electrospinningen_US
dc.subjectGlutaraldehydeen_US
dc.subjectSkin Tissue Engineeringen_US
dc.subjectIn-Vitroen_US
dc.subjectStem-Cellsen_US
dc.subjectSkinen_US
dc.subjectPoly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate)en_US
dc.subjectNanofibersen_US
dc.subjectPoly(Hydroxybutyrate-Co-Hydroxyvalerate)en_US
dc.subjectNanoparticlesen_US
dc.subjectRegenerationen_US
dc.subjectFabricationen_US
dc.subjectCopolymeren_US
dc.titleNatural origin bilayer pullulan-PHBV scaffold for wound healing applicationsen_US
dc.typeArticleen_US

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