Reprogrammed Transcriptome in Rhesus-Bovine Interspecies Somatic Cell Nuclear Transfer Embryos

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dc.WoS.categoriesMultidisciplinary Sciencesen_US
dc.authorid0000-0002-9253-8152en_US
dc.contributor.authorOtu, Hasan Hüseyin
dc.contributor.authorWang, Kai
dc.contributor.authorChen, Ying
dc.contributor.authorLee, Young
dc.contributor.authorLatham, Keith
dc.contributor.authorCibelli, Jose B.
dc.date.accessioned2021-01-18T10:21:30Z
dc.date.available2021-01-18T10:21:30Z
dc.date.issued2011-07-25
dc.description.abstractBackground: Global activation of the embryonic genome (EGA), one of the most critical steps in early mammalian embryo development, is recognized as the time when interspecies somatic cell nuclear transfer (iSCNT) embryos fail to thrive. Methodology/Principal Findings: In this study, we analyzed the EGA-related transcriptome of rhesus-bovine iSCNT 8-to 16-cell embryos and dissected the reprogramming process in terms of embryonic gene activation, somatic gene silencing, and maternal RNA degradation. Compared with fibroblast donor cells, two thousand and seven genes were activated in iSCNT embryos, one quarter of them reaching expression levels comparable to those found in in vitro fertilized (IVF) rhesus embryos. This suggested that EGA in iSCNT embryos had partially recapitulated rhesus embryonic development. Eight hundred and sixty somatic genes were not silenced properly and continued to be expressed in iSCNT embryos, which indicated incomplete nuclear reprogramming. We compared maternal RNA degradation in bovine oocytes between bovine-bovine SCNT and iSCNT embryos. While maternal RNA degradation occurred in both SCNT and iSCNT embryos, we saw more limited overall degradation of maternal RNA in iSCNT embryos than in SCNT embryos. Several important maternal RNAs, like GPF9, were not properly processed in SCNT embryos. Conclusions/Significance: Our data suggested that iSCNT embryos are capable of triggering EGA, while a portion of somatic cell-associated genes maintain their expression. Maternal RNA degradation seems to be impaired in iSCNT embryos. Further understanding of the biological roles of these genes, networks, and pathways revealed by iSCNT may expand our knowledge about cell reprogramming, pluripotency, and differentiation.en_US
dc.fullTextLevelFull Texten_US
dc.identifier.doi10.1371/journal.pone.0022197en_US
dc.identifier.issn1932-6203
dc.identifier.pmid21799794en_US
dc.identifier.scopus2-s2.0-79960742595en_US
dc.identifier.urihttps://hdl.handle.net/11411/3123
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0022197
dc.identifier.wosWOS:000293172900016en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.issue7en_US
dc.language.isoenen_US
dc.nationalInternationalen_US
dc.numberofauthors5+en_US
dc.publisherPublic Library Scienceen_US
dc.relation.ispartofPlos Oneen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMATERNAL MESSENGER-RNASen_US
dc.subjectZYGOTIC GENE ACTIVATIONen_US
dc.subjectSTEM-CELLSen_US
dc.subjectPREIMPLANTATION DEVELOPMENTen_US
dc.subjectMOUSEen_US
dc.subjectOOCYTESen_US
dc.subjectOLIGONUCLEOTIDEen_US
dc.subjectDEGRADATIONen_US
dc.subjectMICROARRAYSen_US
dc.subjectZEBRAFISHen_US
dc.titleReprogrammed Transcriptome in Rhesus-Bovine Interspecies Somatic Cell Nuclear Transfer Embryosen_US
dc.typeArticleen_US
dc.volume6en_US

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