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Cellulose binding protein based generic biosensors
(İstanbul Bilgi Üniversitesi, 2021) Kara, Ali Sinan; Gökdel, Yiğit Dağhan
ABSTRACT: Biosensors are used in various industries. They have great importance in medicine and food industry. Quick prototype production is important for new biosensor production. In this work, a novel method for enzyme immobilization is described to make way for quick biosensor prototype production. Cellulosomes are enzyme complexes that target and degrade cellulosic materials. They utilize a Cellulose binding protein/module (CBM) to attach themselves to the cellulosic material. CBM fused enzymes are used in this work to immobilize enzymes on nanofibril cellulose (NFC) covered screen-printed electrodes. Cellulosic materials are abundant and cheap, thus making them a suitable immobilization material. It is shown in this work that enzymes can be expressed as CBM-fused chimeric proteins to create quick and reliable biosensors.