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    Genotype-Dependent Gene Expression in Strawberry (Fragaria x ananassa) Plants Under High Temperature Stress
    (Springer/Plenum Publishers, 2020) Kesici, Muge; Ipek, Ahmet; Ersoy, Figen; Ergin, Sergul; Gulen, Hatice
    The differences in tolerance to high temperatures were investigated on the basis of gene expressions in two strawberry (Fragaria x ananassaDuch) cultivars which were previously determined as high temperature tolerant (Redlands Hope = R. Hope) and sensitive (Festival). Plants were exposed incrementally to 35, 40, 45, and finally 50 degrees C for 24 h. qRT-PCR analyses were carried out with 19 known sequences from the databases. Protein expression analyses were based on SDS-PAGE results, sequenced and then separated due to their isoelectric points. Expression levels were determined at 35, 40, and 45 degrees C. According to the results, tolerance of 'R. Hope' to high temperature stress can be explained with the coordination of Hsp70, Hsp90, and small heat shock proteins (sHsps) having a vital and supplementary role in stress response. Sensitive cultivar 'Festival' can respond to high temperatures only with the low molecular weight protein and transcripts that do not take a central role in high temperature stress response. Moreover, allergen gene expression triggered by high temperature were detected in both cultivars with different expression levels. The greater expression level in allergen genes observed in the sensitive cultivar 'Festival' under high temperature indicates that there is possibly a negative correlation between expression level in allergen genes and heat stress tolerance. Future studies addressing allergen gene expression under high temperature stress are required to confirm on these findings and to expand on the potential use as a molecular marker in breeding process for enhanced tolerance to high temperature.
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    Molecular and Physiological Responses of Strawberry Plants to Abiotic Stress
    (Cabi Publishing-C A B Int, 2016) Gulen, Hatice; Turhan, Ece; Eris, Atilla
    [Abstract Not Available]
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    Synthesis of molecularly imprinted polymers for specific recognition of ellagic acid
    (IEEE, 2019) Yucel, Necla; Gulen, Hatice; Hatir, Pinar Cakir
    Molecular imprinting technique is based on the fact that functional monomers are formed a complex around the template molecule by covalent or non-covalent interactions, and then these interactions are made stable by using cross-linker. By the removal of the template molecule after the synthesis, the template molecule-specific complementary binding sites are formed in the polymeric network. The ellagic acid molecule, which is found in the pomegranate fruit, is known to have anti-inflammatory, antiallergic, anti-cancer and antimicrobial properties. In this study, it is aimed to synthesize ellagic acid imprinted polymer (MIP) for isolation of ellagic acid from pomegranate fruit. 4-vinylpyridine was used as functional monomer, ethylene glycol dimethacrylate as crosslinker, and Tetramethylethylenediamine (TEMED)/Potassium persulfate (KPS) system as initiator. Molecularly imprinted polymers were synthesized by thermal polymerization. The characterization of the synthesized polymers was done by Fourier-transform infrared spectroscopy (FTIR). The ellagic acid recognition properties of MIP and nonimprinted polymers (NIPs) were tested, dissociation constants were calculated as 7.0 mu M for MIP and 9.5 mu M with lower affinity for NIP. In the light of the findings, it was concluded that MIP can recognize ellagic acid better than NIP.