Multiscanning mode laser scanning confocal microscopy system

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dc.WoS.categoriesComputer Science, Artificial Intelligence; Engineering, Electrical & Electronicen_US
dc.authorid0000-0003-4634-4733en_US
dc.authorid0000-0002-7433-3823en_US
dc.authorid0000-0001-6920-2565en_US
dc.authorid0000-0003-3104-7410en_US
dc.contributor.authorAktürk, Mert
dc.contributor.authorGümüş, Gökhan
dc.contributor.authorSarıoğlu, Baykal
dc.contributor.authorGökdel, Y. Dağhan
dc.date.accessioned2021-02-24T07:31:24Z
dc.date.available2021-02-24T07:31:24Z
dc.date.issued2019
dc.description.abstractIn this paper, a table-top, reflective mode, laser scanning confocal microscopy system that is capable of scanning the target specimen alternately through various scanning devices and methods is proposed. We have developed a laser scanning confocal microscopy system to utilize combinations of various scanning devices and methods and to be able to characterize the optical performance of different scanners and micromirrors that are frequently used in scanning microscopy systems such as multiphoton microscopy, optical coherence tomography, or confocal microscopy. By integrating the scanner to be characterized on the same optical path with a galvanometric scan mirror, which is the conventional benchmarking scanning unit in a typical scanning microscope, we obtain two major advantages: (1) microscopy images are automatically acquired from the same location on the target specimen without having any timeconsuming alignment problem and accordingly provide a high-quality optical comparison opportunity, and (2) it totally eliminates the utilization of a second scanning microscopy to benchmark the performance of the scanner-based system and considerably reduces the time spent for imaging, which is a crucial factor for a freshly excised tissue, especially under a fluorescence microscope. The system is composed of a 658 nm laser source, collimation optics, a 2D galvanometer, a 2D polymer micro-scanner, an objective lens with a numerical aperture of 0.40, a 100 mu m pinhole, a PMT, a DAQ card and peripheral electronics as well as a Matlab software that simultaneously controls the system through a personal computer. Prototype of the proposed flexible LSCM system is first optically characterized using a USAF resolution target. Subsequently, we provided images of red blood and bacteria cells to demonstrate the systems' capability for clinical diagnostics. It is reported that maximum FOV and lateral resolution of the proposed LSCM are measured to be 420 mu m x 360 pm and 1 mu m with galvanometer and, and 117 mu m x 117 mu m and 3.2 mu m with the polymer scanner unit, respectively.en_US
dc.fullTextLevelFull Texten_US
dc.identifier.doi10.3906/elk-1807-268en_US
dc.identifier.issn1303-6203
dc.identifier.issn1300-0632
dc.identifier.scopus2-s2.0-85065815472en_US
dc.identifier.trdizinid336600en_US
dc.identifier.urihttps://hdl.handle.net/11411/3278
dc.identifier.urihttps://doi.org/10.3906/elk-1807-268
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/336600en_US
dc.identifier.wosWOS:000463355800030en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.issue2en_US
dc.language.isoenen_US
dc.nationalInternationalen_US
dc.numberofauthors4en_US
dc.pages1081-1093en_US
dc.publisherTubitak Scientific & Technical Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal Of Electrical Engineering And Computer Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectLaser scanning confocal microscopyen_US
dc.subjectskin canceren_US
dc.subjectpolymer scanneren_US
dc.subjectgalvanometric scan mirrorsen_US
dc.subjectraster scanningen_US
dc.subjectLissajous scanningen_US
dc.subjectbenchmarkingen_US
dc.titleMultiscanning mode laser scanning confocal microscopy systemen_US
dc.typeArticleen_US
dc.volume27en_US

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