Determining T and B Cell development by TREC/KREC analysis in primary immunodeficiency patients and healthy controls

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dc.authoridNG, YUK YIN/0000-0001-9755-6045|ÖZEN, Ahmet Haris Selçuk/0000-0002-3251-3236|Ozbek, Ugur/0000-0001-5319-0547|Kiykim, Ayca/0000-0001-5821-3963|Ar, Muhlis Cem/0000-0002-0332-9253|Firtina, Sinem/0000-0002-3370-8545|Beken, Serdar/0000-0002-8609-2684|Kolukisa, Burcu/0000-0002-2399-8630|OGULUR, ISMAIL/0000-0001-8282-7762|Temel, Sehime G/0000-0002-9802-0880|Aydin Sayitoglu, Muge/0000-0002-8648-213X|Onder, Gizem/0000-0003-4933-8048|Altindirek, Didem/0000-0001-5068-2968
dc.authorwosidFırtına, Sinem/GSD-3891-2022
dc.authorwosidYilmaz, Hulya/D-4424-2019
dc.authorwosidNG, YUK YIN/AAG-7977-2020
dc.authorwosidAydiner, Elif/HKN-1599-2023
dc.authorwosidÖZEN, Ahmet Haris Selçuk/ABB-8708-2020
dc.authorwosidOzbek, Ugur/C-9513-2017
dc.authorwosidKiykim, Ayca/J-6400-2017
dc.contributor.authorSenturk, Gizem
dc.contributor.authorNg, Yuk Yin
dc.contributor.authorEltan, Sevgi Bilgic
dc.contributor.authorBaser, Dilek
dc.contributor.authorOgulur, Ismail
dc.contributor.authorAltindirek, Didem
dc.contributor.authorFirtina, Sinem
dc.date.accessioned2024-07-18T20:47:35Z
dc.date.available2024-07-18T20:47:35Z
dc.date.issued2022
dc.departmentİstanbul Bilgi Üniversitesien_US
dc.description.abstractT cell receptor excision circles (TRECs) and kappa-deleting excision circles (KRECs) are DNA fragments potentially indicative of T and B cell development, respectively. Recent thymic emigrants (RTEs) are a subset of peripheral cells that may also represent thymic function. Here, we investigated TREC/KREC copy numbers by quantitative real-time PCR in the peripheral blood of patients with primary immunodeficiencies (PIDs, n = 145) and that of healthy controls (HCs, n = 86) and assessed the correlation between RTEs and TREC copy numbers. We found that TREC copy numbers were significantly lower in children and adults with PIDs (P < .0001 and P < .002, respectively) as compared with their respective age-matched HCs. A moderate correlation was observed between TREC copies and RTE numbers among children with PID (r = .5114, P < .01), whereas no significant correlation was detected between RTE values and TREC content in the HCs (r = .0205, P = .9208). Additionally, we determined TREC and KREC copy numbers in DNA isolated from the Guthrie cards of 200 newborns and showed that this method is applicable to DNA isolated from both peripheral blood samples and dried blood spots, with the two sample types showing comparable TREC and KREC values. We further showed that RTE values are not always reliable markers of T cell output. Although additional confirmatory studies with larger cohorts are needed, our results provide thresholds for TREC/KREC copy numbers for different age groups.en_US
dc.identifier.doi10.1111/sji.13130
dc.identifier.issn0300-9475
dc.identifier.issn1365-3083
dc.identifier.issue3en_US
dc.identifier.pmid34951041en_US
dc.identifier.scopus2-s2.0-85122142217en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1111/sji.13130
dc.identifier.urihttps://hdl.handle.net/11411/7860
dc.identifier.volume95en_US
dc.identifier.wosWOS:000737540800001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofScandinavian Journal of Immunologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectKappa-Deleting Excision Circleen_US
dc.subjectNewborn Screeningen_US
dc.subjectPrimary İmmune Deficiencyen_US
dc.subjectRecent Thymic Emigrantsen_US
dc.subjectT Cell Receptor Excision Circleen_US
dc.subjectThymic Emigrantsen_US
dc.subjectExcision Circlesen_US
dc.subjectNewbornen_US
dc.subjectRecombinationen_US
dc.subjectDiseasesen_US
dc.subjectReceptoren_US
dc.titleDetermining T and B Cell development by TREC/KREC analysis in primary immunodeficiency patients and healthy controls
dc.typeArticle

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