Intra- and Extra-cellular Thiol-Disulfide Homeostasis in Blood of Patients with Alcohol Use Disorder
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Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
Pleiades Publishing Inc
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
Alcohol use disorder (AUD) is the loss of a person's quality of life due to increased alcohol consumption and the failure of alcohol consumption control. In this study, the intra- and extracellular thiol-disulfide levels in people with AUD were compared with age-matched healthy controls. Forty male patients diagnosed with AUD and thirty healthy male individuals were included in the study. In addition to psychiatric and sociodemographic tests routine biochemical evaluations have been performed. The alcohol group had significantly higher Michigan Alcohol Screening Test (MAST), Beck Depression Inventory (BDI), and Beck Anxiety Inventory (BAI) scores compared to the control group. While there was a decrease in the intracellular GSH and extracellular native thiol levels in the blood of the alcohol group, GSSG and disulfide levels were increased, respectively. Subsequently, while there was an increase in the intracellular GSSG/GSH content levels in the alcohol group compared to the control group, the ratios of GSH/GSH+GSSG were decreased. These changes, indicative of oxidative stress, were observed both in blood serum and erythrocytes. Also, there was an increase in alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyltransferase (GGT) mean corpuscular volume (MCV), C-reactive protein (CRP) levels in the alcohol group in routine biochemical tests, while total protein, folic acid, ferritin and hemoglobin (HGB) levels were decreased. Our results suggest that increased intra- and extracellular GSSG/GSH % (disulfide/native thiol %) may serve as an indicator of developing oxidative stress in individuals with AUD.
Description
Keywords
Alcohol Use Disorders, Disulfides, Glutathione, Oxidative Stress, Thiols, Oxidative Stress, Glutathione, Inventory, Abuse
Journal or Series
Journal of Evolutionary Biochemistry and Physiology
WoS Q Value
Q4
Scopus Q Value
Volume
57
Issue
4
