A Comparison of GAPDH and ACTB As Internal Control for Gene Expression Studies in Different Cancer Cell Lines

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dc.authorid0000-0001-9226-6444
dc.contributor.authorKose, Tugba
dc.date.accessioned2026-04-04T18:56:10Z
dc.date.available2026-04-04T18:56:10Z
dc.date.issued2024
dc.departmentİstanbul Bilgi Üniversitesi
dc.description.abstractHousekeeping genes are used as internal controls to normalize the expression of target genes in gene expression studies. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and Actin beta (ACTB) are frequently preferred as housekeeping genes in gene expression studies. Due to the general alterations in the gene expression pattern in cancer cases, the selection of the appropriate housekeeping genes for these studies are challenging. In this study, we aimed to analyze the expression of the well-known housekeeping genes GAPDH and ACTB in 6 different cancer cell lines. Furthermore, the relative gene expression of the selected target gene (Tissue inhibitor of metalloproteinases 2-TIMP2) was normalized separately using GAPDH and ACTB and the obtained results were compared with each other. Finally, the stability of GAPDH and ACTB was analyzed using the in-silico tool, Bestkeeper. As a result of the study, it is found that the expression of GAPDH and ACTB were significantly different in the Jurkat (p< 0.01), MOLT4 (p< 0.05), REH (p< 0.001) and HT29 (p< 0.001) cell lines. Based on this finding, significantly different relative target gene expression values were obtained in different cell lines depending on whether the selected housekeeping gene was GAPDH or ACTB. In addition, GAPDH was found to show less variation among the samples used in all cell lines and more stability based on Bestkeeper analysis. These results support that the appropriate housekeeping gene selection, especially in cancer cell lines, may be an effective factor in obtaining accurate results for the studies in the field provide guidance to researchers.
dc.identifier.doi10.4999/uhod.247615
dc.identifier.doi10.4999/uhod.247615
dc.identifier.endpage147
dc.identifier.issn1306-133X
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85208413516
dc.identifier.scopusqualityQ4
dc.identifier.startpage140
dc.identifier.urihttps://doi.org/10.4999/uhod.247615
dc.identifier.urihttps://hdl.handle.net/11411/10729
dc.identifier.volume34
dc.identifier.wosWOS:001342121900004
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherAkad Doktorlar Yayinevi
dc.relation.ispartofUhod-Uluslararasi Hematoloji-Onkoloji Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20260402
dc.snmzKA_Scopus_20260402
dc.subjectCancer Cell Lines
dc.subjectGene Expression
dc.subjectHousekeeping Genes
dc.subjectGapdh
dc.subjectActb
dc.titleA Comparison of GAPDH and ACTB As Internal Control for Gene Expression Studies in Different Cancer Cell Lines
dc.typeArticle

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